Ohio State - Nationwide Children's Hospital - Research For Muscle Biology and Disease

Dawn S. Chandler, Ph.D.Dawn S. Chandler
Associate Professor

The Ohio State University School of Medicine
Department of Pediatrics
Nationwide Children's Research Institute
Investigator, Center for Childhood Cancer
700 Children's Drive, Rm. WA5023
Columbus, OH  43205

Phone: (614) 722-5598
Fax: (614) 722-5895
Email: Dawn.Chandler@nationwidechildrens.org
Website: http://www.ccri.net

Education & Training:
University of Texas Health Science Center, Houston, TX, 1998 Ph.D. in Biomedical Sciences
M.D. Anderson Cancer Center, Houston, TX, 2002 Postdoctoral Fellow Human Genetics
M.D. Anderson Cancer Center, Houston, TX, 2004 Postdoctoral Fellow Cancer Genetics

Research Interest:
Our lab is interested in the regulation of pre-mRNA splicing and how disruption of this regulation can lead to pediatric diseases such as cancer.  Current work in our lab elucidates alternative splicing as a novel mechanism by which cellular injury can control the activity of p53 and how changes in the regulation of splicing can lead to tumorigenesis.  The transcription factor p53 is known to induce G1 arrest of the cell cycle and/or apoptosis. MDM2 is one of the most critical regulators of p53.  Using in vitro biochemical assays and genetically engineered mouse models we are currently investigating differential RNA splicing of both the MDM2 and p53 pre-mRNAs and investigating the roles of each in normal cell function as well as disease.

Another pediatric disease Proximal Spinal Muscular Atrophy (SMA), the leading genetic cause of infant mortality in humans, is in part due to a mutation that affects splicing of a duplicated gene that controls neuronal growth (SMN2).  We are interested in generating viable mouse models for human SMA with the long-term goal of testing candidate therapies that target the human SMN2 gene.  To do this, we are generating mouse lines that will be utilized to answer many questions pertaining the therapeutic possibilities of SMN replacement, splicing correction by drug or antisense treatment, and the correct timing of such therapies.

Selected Publications:

  • Chandler, D.S., Singh, R.K., Caldwell, L.C., Bitler, J.L., and Lozano, G. Genotoxic stress induces coordinately regulated alternative splicing of the p53 modulators MDM2 and MDM4.  Cancer Research, 66: 9502-9508, 2006.