Noah Weisleder, Ph.D.
The Ohio State University Wexner Medical Center
473 W. 12th Ave
Davis Heart and Lung Research Institute, 611A
Columbus, OH 43210-1252
Phone: (614) 292-5321
Fax: (614) 247-7799
Education & Training:
Worcester Polytechnic Institute, 1996, B.S. in Biotechnology and Molecular Biology
Baylor College of Medicine, 2003, Ph.D. in Molecular and Cellular Biology
Robert Wood Johnson Medical School, 2008, Postdoctoral Fellow in Physiology and Biophysics
Our laboratory studies several different aspects of skeletal muscle and cardiovascular physiology, principally focusing on changes in plasma membrane repair, cellular metabolism and calcium homeostasis in normal physiology and disease states. We are currently examining how aberrant store-operated calcium handling can contribute to the progression of muscular dystrophy and developing methods to block this calcium entry. Additionally, efforts in heart failure models will determine the mechanisms that contribute to uncoupling of intracellular calcium homeostasis in the failing cardiomyocytes. Further studies examine the role of tripartite motif (TRIM) family proteins in the membrane repair process. Membrane repair is a conserved cellular process where intracellular vesicles actively patch membrane disruptions to allow survival of the cell. While relevant in normal physiology, disruption of this process results in diseases, including muscular dystrophy, ischemic damage to the heart and neurodegeneration. Our group is examining the role of novel TRIM family proteins in regulating membrane repair and how these can be targeted as therapeutic interventions in a number of different diseases.
- Zhao X, Moloughney JG, Zhang S, Komazaki S, Weisleder N. Orai1 mediates exacerbated Ca2+ entry in dystrophic skeletal muscle. PLoS One. 2012; http://dx.plos.org/10.1371/journal.pone.0049862
- Weisleder N, Takizawa N, Lin P, Wang X, Cao C, Zhang Y, Tan T, Ferrante C, Zhu H, Chen PJ, Yan R, Sterling M, Zhao X, Hwang M, Takeshima M, Cai C, Cheng H, Takeshima H, Xiao RP, Ma J. Recombinant MG53 protein modulates therapeutic cell membrane repair in treatment of muscular dystrophy. Sci Transl Med. 2012 Jun 20;4(139):139ra85.
- He B, Tang RH, Weisleder N, Xiao B, Yuan Z, Cai C, Zhu H, Lin P, Qiao C, Li J, Mayer C, Li J, Ma J, Xiao X. Enhancing Muscle Membrane Repair by Gene Delivery of MG53 Ameliorates Muscular Dystrophy and Heart Failure in δ-Sarcoglycan-deficient Hamsters. Mol Ther. 2012 Apr;20(4):727-35.
- Touchberry CD, Elmore CJ, Nguyen TM, Andresen JJ, Zhao Xiaoli, Orange M, Weisleder N, Brotto M, Claycomb WC, Wacker MJ. Store-operated calcium entry is present in HL-1 cardiomyocytes and contributes to resting calcium. Biochem Biophys Res Commun. 2011 Dec 9;416(1-2):45-50. Epub 2011 Nov 6. PMID: 22130850
- Tjondrokoesoemo A, Park KH, Ferrante C, Komazaki S, Lesniak S, Brotto M, Ko JK, Zhou J, Weisleder N, Ma J. Disrupted membrane structure and intracellular Ca2+ signaling in adult skeletal muscle with acute knockdown of Bin1. PLoS One. 2011;6(9):e25740. PMID: 22079292
- Yi J, Ma C, Li Y, Weisleder N, Rios E, Ma J, Zhou J. Mitochondrial calcium uptake regulates rapid calcium transients in skeletal muscle during excitation-contraction (E-C) coupling. J Biol Chem. 2011 Sep 16;286(37):32436-43.
- Thornton AM, Zhao X, Weisleder N, Brotto LS, Bougoin S, Nosek TM, Reid M, Hardin B, Pan Z, Ma J, Parness J, Brotto M. Store-Operated Ca2+ Entry (SOCE) Contributes to Normal Skeletal Muscle Contractility in young but not in aged skeletal muscle. Aging 2011 Jun;3(6):621-34.
- Weisleder N, Takeshima H, Ma J. Mitsugumin 53 (MG53) facilitates vesicle trafficking in striated muscle to contribute to cell membrane repair. Communicative & Integrative Biology. 2009 March/April 2(3):1-2.
- Fang H, Chen M, Ding Y, Shang W, Xu J, Zhang X, Zhang W, Li K, Xiao Y, Gao F, Shang S, Li JC, Tian XL, Wang SQ, Zhou J, Weisleder N, Ma J, Ouyang K, Chen J, Wang X, Zheng M, Wang W, Zhang X, Cheng H. Imaging superoxide flash and metabolism-coupled mitochondrial permeability transition in living animals. Cell Res. 2011 Sep;21(9):1295-304
- Landstrom AP, Kellen CA, Dixit SS, van Oort RJ, Garbino A, Weisleder N, Ma J, Wehrens XH, Ackerman MJ. Junctophilin-2 Expression Silencing Causes Cardiocyte Hypertrophy and Abnormal Intracellular Calcium-Handling. Circ Heart Fail. 2011 Mar 1;4(2):214-23.